Functional Domains in the Heavy - Chain Region of Factor XI : A High Molecular
نویسندگان
چکیده
To probe the molecular interactions of factor Xl we have prepared two monoclonal antibodies (MoAbs; 5F7 and 3C1 ), each of which binds the heavy chain of reduced and alkylated factor Xla. Competitive solid phase radioimmunoassay (RIA) binding studies revealed that 5F7 and 3C1 are directed against different epitopes within factor Xl. One antibody (5F7) blocked the surface-mediated proteolytic activation of factor Xl and its binding to HMW kininogen, but had no effect on factor-Xla-.catalyzed factor IX activation. The other antibody (3C1 ) is a competitive inhibitor of factor-IX activation by factor Xla, but blocked factor-XI binding to HMW kininogen only at 1,000-fold higher concentration than 5F7. Moreover, HMW kininogen had no effect on the kinetics of factor-Xla-catalyzed fac-
منابع مشابه
Functional domains in the heavy-chain region of factor XI: a high molecular weight kininogen-binding site and a substrate-binding site for factor IX.
To probe the molecular interactions of factor XI we have prepared two monoclonal antibodies (MoAbs; 5F7 and 3C1), each of which binds the heavy chain of reduced and alkylated factor XIa. Competitive solid phase radioimmunoassay (RIA) binding studies revealed that 5F7 and 3C1 are directed against different epitopes within factor XI. One antibody (5F7) blocked the surface-mediated proteolytic act...
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